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Home :: HPLC Columns :: SIELC Technologies :: Primesep Series (Mixed-Mode)
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Primesep Series (Mixed-Mode)

SIELC Primesep HPLC columns are designed for mixed-mode separations and capable of separating a tremendous range of compounds by different separation modes based only on the mobile phase selection. With an embedded ion-pairing group, the columns require no ion-pairing reagents in the mobile phase to retain and separate ionizable polar compounds. These columns have shown to efficiently separate organic and inorganic ions in ion-exchange and ion-exclusion modes. The columns can be used to provide efficient separation in normal phase, reverse phase, and polar organic modes. Different modes of separation offer different column selectivity.

Acidic
Basic
Complex
Phenyl

Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent. Primesep A (Strongest acidic phase), Primesep 100 (Strong acidic phase), Primesep 200 (Medium acidic phase).

Columns with positively charged functional groups. Primesep B (Strong basic column) for use with TFA, phosphoric or perchloric acid solutions in a mobile phase. Primesep B2 (Weak basic column) for ammonium acetate buffered solution.

Column with unique unparallel selectivity. Primesep C Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions.

Three interactions within one column, Reverse Phase, pi-pi, strong cation exchange Primesep P choose for separation of structural isomers of aromatic compounds.
Primesep Brochure Primesep Separation of Ions Brochure Primesep Pharmaceutical HPLC Analysis Brochure
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