ProntoSIL C30 Series

ProntoSIL C30 HPLC columns are particularly recommended for the separation of hydrophobic, long-chain, structural isomers.They are often a better alternative to normal phase columns for the separation of isomers since they are not as sensitive as normal phase columns to water content of the mobile phase and are not as susceptible to column fouling. ProntoSIL greater shape selectivity compared to C18 phases due to their rigid, highly ordered C30 alkyl groups. This shape selectivity advantage makes them the ideal HPLC column to use for the separation of carotenoids and tocopherol derivatives. For best performance, C30 columns should be used at ambient temperature or lower. At elevated temperatures, the C30 alkyl chains will become less ordered and lose their shape selectivity. In addition, C30 phases should be used with mobile phases containing at least 20% organic modifier to avoid phase collapse. Although you can expect lower plate count on C30 phases compared to C18 phases, the ability of the C30 phases to separate isomers that are not possible to separate on C18 phases make them an attractive choice for many applications. C30 phases have been successfully used for the analysis of plant extracts, food, and biological tissues that contain structurally different carotenoids and mixtures of geometricisomers. They are also commonly used for separating stereoisomers of vitamin E and vitamin A, and for the separation of PAHs.