Inspire HILIC 100Å - 5µm

Dikma Inspire™ columns are engineered with high purity raw silica, proprietary bonding techniques, tightly controlled manufacturing processes, and column packing procedures that provide today’s chromatographic laboratories with HPLC columns of unrivaled performance.

HILIC is normal phase chromatography of polar and ionic compounds under RP conditions. It combines the characteristics of three methods in LC – RP, NP, and IC. Inspire HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of polar compounds onto the stationary phase for increased retention. Separation is achieved through the partitioning of polar solutes from the high concentration, water-miscible, organic mobile phase into the hydrophilic surface environment. Polar solutes exhibit increased retention, and elute in the order of increasing hydrophilicity. HILIC is ideal for separating and retaining polar compounds that may not retain on traditional RP packings. Unlike reversed-phase columns, Inspire HILIC columns retain highly polar compounds with only small amounts of water in the mobile phase. These more volatile mobile phases increase sensitivity with microbore applications.

Dikma Chromatography Catalog