Agilent Advanced silica production and silane bonding techniques allow for the production of material with exceptional base-deactivated characteristics. Agilent MonoChrom columns provide excellent chromatography of organic amines, organic acids, amphoteric compounds, and neutral compounds. Agilent MonoChrom
was developed to complement the selectivity of Agilent’s other base deactivated columns while improving the base-deactivated properties.
Agilent MonoChrom MS column is bonded with a unique fluorinated phenyl phase that retains basic compounds by either reverse phase or normal phase mode.
This phase was specifically designed for ESI/MS detection where high organic eluent (i.e. normal phase) result in increased retention of bases. Sensitivity for
ESI/MS is greatly enhanced under these eluent conditions. Common organic solvents (acetonitrile, methanol, etc.) used in HPLC have lower surface tension
and higher volatility than aqueous solvents. The lower surface tension and higher volatility produces higher MS signals due to more efficient desolvation
in ESI. For optimum MS signal, mobile phases with high organic/aqueous ratios are needed for a more efficient desolvation process, thereby enhancing
