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Made of narrow-dispersed, spherical, low cross-linking polystyrene/divinylbenzene (PS/DVB) particles. Surfaces functional groups are sulfonic acid (-SO3H), or metal ions, such as calcium (Ca+2). The PS/DVB support has cross-linking degree of 5%, 8% and 10%. The particle size selection is 3, 5 and 10 µm for analytical and preparative separations of carbohydrates, organic acids, peptides, and nucleic acids.