COSMOSIL HIC 300Å (Hydrophobic Interaction Chromatography)

COSMOSIL 5HIC is designed for one step desalting and separation of proteins. Hydrophobic interaction chromatography (HIC) is an effective method for purification and separation of proteins (especially enzymes) based on differences in their surface hydrophobicity. Since this method does not use organic solvents like reversed phase chromatography, there is only a little loss in enzyme activity and the tertiary structure of proteins. A buffer with high salt concentration, usually 1-2 mol/l of (NH4)2SO4, is used as an initial mobile phase for adsorption of samples to a weakly hydrophobic stationary phase. The elution is done with a decreasing salt gradient.

COSMOSIL HIC Applications