Hamilton RCX-30 100Å (Carbohydrate Separation)

The Hamilton RCX-10 and RCX-30 carbohydrate analysis columns are designed for the isocratic or gradient separation of carbohydrates. The exchange capacity of the RCX-10/RCX-30 is greater than that of the PRP-X100, leading to characteristics better suited for the separation of carbohydrates. Simple samples with two or three carbohydrates can be quickly separated isocratically, while more complex samples require gradient elution to fully resolve all the analytes of interest. When an isocratic method is used with a conductivity, refractive index, ultraviolet, or pulsed amperometric detector (PAD), mono and disaccharides such as glucose, fructose, sucrose and lactose can be quickly determined.

To utilize the full potential of the RCX-10 column (e.g., gradient separations) a Pulsed Amperometric Detector (PAD) is recommended. The PAD allows utilization of either gradient or isocratic elution for the separation of carbohydrates in foods or food products. A typical mobile phase is sodium hydroxide and sodium acetate. When the concentration of these mobile phases is varied, a variety of samples can be separated. Separation of carbohydrates with the RCX-10 or RCX-30 at basic pH is possible since each carbohydrate carries a different negative charge at basic pH. RCX-30 carbohydrate analysis columns provide longer sample retention than RCX-10 columns and better resolution of complex samples like the six constituent monosaccharides of glycoconjugates. It is the increased exchange capacity of the RCX-30 that gives it these characteristics as compared to the RCX-10.