Hamilton PRP-X500 (Anion Exchange USP L23)

Hamilton PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns. The non-porous nature of the packing material improves mass-transfer, shortening run times and improving resolution. Both fast separations and good sample capacity are achievable with PRP-X500 columns. A separation of four protein standards at 0.2 mg in less than three minutes is possible with a short analytical 50 x 4.6 mm HPLC column. Recovery of sample is excellent with PRP-X500 and the support’s limited permeability prevents proteins from entering the pores and unfolding, which causes peak ghosting. The superficially porous properties shorten the diffusion path of the analyte, resulting in sharp sample bands.