Agilent Bio MAb Series (Monoclonal Antibodies Separation)

Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies. Bio MAb columns are available in 1.7μm, 3μm, 5μm, and 10μm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column.

• A packing support composed of a rigid, spherical, highly cross-linked polystyrene divinylbenzene (PS/DVB) non-porous bead
• Particles grafted with a hydrophilic, polymeric layer, virtually eliminating non-specific binding of antibody proteins
• A different process is used to layer the weak cation-exchange phase to the particle making it a higher density than the Agilent Bio WCX column particles
• Specifically designed for the separation of charge isoforms of monoclonal antibodies

Application Note - Analysis of Intact and C-terminal Digested IgG1 on an Agilent Bio MAb 5µm Column

Application Note - pH Gradient Elution for Improved Separation of Monoclonal Antibody Charge Variants