SIELC Promix AP 300Å Series

Promix is an alternative chromatography technology for efficient resolution of peptides and proteins. The technology is based on a combination of two interactions – hydrophobic and ionic.

Promix AP is a column designed for short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides on this column. Use a buffer with a neutral or slightly acidic pH (4.5 – 7) in the beginning of the gradient, and use a more acidic buffer (pH 2 – 3.5) in the end of the gradient elution.

Promix Brochure