SIELC Primesep HPLC columns are designed for mixed-mode separations and capable of separating a tremendous range of compounds by different separation modes based only on the mobile phase selection. With an embedded ion-pairing group, the columns require no ion-pairing reagents in the mobile phase to retain and separate ionizable polar compounds. These columns have shown to efficiently separate organic and inorganic ions in ion-exchange and ion-exclusion modes. The columns can be used to provide efficient separation in normal phase, reverse phase, and polar organic modes. Different modes of separation offer different column selectivity.
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Columns with negatively charged functional groups due to the embedded anionic ion-pairing reagent. Primesep A (Strongest acidic phase), Primesep 100 (Strong acidic phase), Primesep 200 (Medium acidic phase). |
Columns with positively charged functional groups. Primesep B (Strong basic column) for use with TFA, phosphoric or perchloric acid solutions in a mobile phase. Primesep B2 (Weak basic column) for ammonium acetate buffered solution. |
Column with unique unparallel selectivity. Primesep C Combined reverse-phase and complex formation properties for interaction with amines, sulfonium, phosphonium and metal ions. |
Three interactions within one column, Reverse Phase, pi-pi, strong cation exchange Primesep P choose for separation of structural isomers of aromatic compounds. |
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