ZORBAX StableBond 300SB-C18 300Å - 3.5µm

Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.

The Stablebond C18 bonding is a sterically-protected C18 functionality (diisobutyl n-octadecylsilane) for long column lifetimes when using acidic mobile phases. This is important for the analysis of peptide fragments from protein digests and small proteins.