Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers. These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80°C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
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