EVSecond (Exosomes Purification Column)

EVSecond is a size exclusion chromatography open column optimized for effective purification of exosomes. Highly-purified exosomes can be easily collected from serum, plasma, or cell culture supernatant. (EVSecond：Extracellular Vesicle Isolation by Size Exclusion Chromatography on Drip Column). A large amount of free proteins, metabolites, and nucleotides are involved in serum samples. Insufficient purification of exosomes often causes co-detection of non-exosomal components, leading to incorrect quantification results in omics studies. Exosomes were isolated from 100 µL, 200 µL, 300 µL, or 700 µL of human serum using EVSecond method. Exosomes were clearly separated from serum free proteins such as albumin or immunoglobulins.

• Simple gravity-flow handling without ultracentrifugation
• EVSecond-purified exosomes possess efficient purity for comprehensive miRNA, proteome, and metabolome analysis
• Exosomes are gently eluted in PBS without structural damage, allowing readministration experiments of collected exosomes to cells or animals
• EVSecond L70 can used at room temperature and the preparation of column is now much easier