The AdvanceBio SEC 1.9 µm column is designed to improve resolution and provided faster separation of mAb aggregates and fragments by using both HPLC and UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility and stability, as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 200 Å 1.9 μm column is intended to meet the need for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.