Mixed mode column technology combining reversed-phase, anion exchange and cation exchange functionality on a single support
The Thermo Scientific™ Acclaim™ Trinity™ P1 HPLC column is designed with Nanopolymer Silica Hybrid (NSH) technology, which results in a multimode surface chemistry ideal for the simultaneous separation of drugs and their counterions. The surface chemistry concurrently provides reversed-phase, cation exchange, and anion exchange functionalities. The result is maximum flexibility in method development. Separations can be optimized easily by adjusting the chromatographic parameters (mobile phase pH, ionic strength, and organic strength).
Ideal selectivity for simultaneous separation of API and counterion
Adjustable selectivity by mobile phase ionic strength, electrolyte type, pH, and organic solvent
Low bleed; compatible with MS, CAD and ELSD
Retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents
Greater flexibility in method development: each retention mechanisms can be controlled independently
