ZORBAX GF-250/450 Series (SEC)

Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are ideal for size separations of proteins and other biomolecules. The separation range is Mw 4,000-900,000g/mol for globular proteins when using GF-250 and GF-450 columns in series.

The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8.

The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a highly efficient, rugged and reproducible size exclusion column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Some common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins.