Hamilton HC-75 H+ Form

Hamilton HC-75 columns separate compounds through ligand exchange. The 8% cross-linked Hamilton HC-75 uses ligand exchange as the primary mechanism of separation. The different forms of the Hamilton HC-75 (Hydrogen H⁺, Calcium Ca²⁺ and Lead PB²⁺) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.

The Hamilton HC-75 columns use water as a mobile phase (gradients and salts are not required), which simplifies eluent preparation and minimizes cost. This mobile phase characteristic also lends these columns to detection techniques such as evaporative light scattering detection and mass spectrometry. A very versatile column family, the HC-75 group can be used with up to 40% acetonitrile and can be regenerated to help restore performance.