Hamilton HC-40 100Å (Ligand Exchange for Carbohydrates)

Hamilton HC-40 columns separate compounds through size exclusion. The 4% cross-linked HC-40 uses size exclusion as the primary mechanism of separation. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later. The Hamilton HC-40 columns use water as a mobile phase (gradients and salts are not required), which simplifies eluent preparation and minimizes cost. This mobile phase characteristic also lends these columns to detection techniques such as evaporative light scattering detection and mass spectrometry.