Shodex MSpak GF-4A (On-line Surfactant Removal)

Shodex MSpak GF-4A is a multi-mode pretreatment Column for Column Switching Method. It can be used for size exclusion chromatography and reverse phase chromatography. Because the adsorption of the protein is a little, it can be used also for the medicine concentrate in the sample including the protein.

Surfactants are often used to solubilize cell membrane and/or to prevent peptide/protein to be adsorbed on the container surface. However, the presence of surfactant may deteriorate LC column and LC/MS system, results in poor repeatability. By using MSpak GF-4A, surfactant can be removed prior to sample being introduced to the analytical column. Therefore provides good repeatable analysis and reduces the time required for sample preparation. The following examples of surfactants can be removed by GF-4A.

• Triton X-100
• Sodium dedecyl sulfate (SDS)
• Sodium dodecylbenzenesulfonate (SDBS)
• Cetyltrimethyl ammonium bromide (CTABr)