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Agilent HC-C18(2) and TC-C18(2) columns can be used for method development of acidic, basic and neutral analytes. The TC-C18(2) column has a 12% carbon load, optimized for retention of more polar analytes for use with gradient methods that start at very low aqueous down to 5% aqueous - with excellent results. Typical samples include complex natural products and traditional medicines. Other typical applications would include those with samples dissolved or prepared in water, such as the analysis of additives or sweeteners in beverages. The TC-C18(2) column is shipped with the new Agilent 1120 Compact LC.
The HC-C18(2) column has a higher carbon load 17% - and can be more retentive for moderately polar and non-polar analytes. This column would be a better choice for method development or routine applications where the sample is dissolved or prepared in organic solvents.
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