Agilent Hi-Plex Ca (Duo) uses a duo-calcium counter-ion to provide selectivity differences through its ability to interact with the hydroxyl group. Hi-Plex Ca (Duo) typically uses isocratic conditions with water as the eluent and temperature as the main variable for resolution control. USP L classification L19. Applications are oligosaccharides with a Dp <5 with monosaccharides.
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