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Due to the complex nature of water soluble polymers, it is often necessary to modify the eluent in order to avoid sample-to-sample and sample-to-column interactions which can result in poor aqueous SEC separations. The excellent stability of the PL aquagel-OH packing material allows the eluent to be tailored to suit the polymer, while retaining the high column efficiency. For ionic interactions, the eluent can be modified by the addition of salt and/or the adjustment of pH. For water soluble polymers with a hydrophobic character, only the addition of a weak organic solvent (methanol) is required to inhibit hydrophobic interactions.
PL aquagel-OH Analytical Columns are available with mixed and individual pore sizes, and 5, 8 and 15 μm particle sizes, to cover a very wide range of molecular weights. PL aquagel-OH Preparative Columns for rapid and convenient scale-up from analytical separations. The columns are packed with the same robust macroporous particles as the analytical column range
Agilent aqueous and polar GPC/SEC Brochure